利用重测序技术获取转基因植物T-DNA插入位点

徐纪明,胡晗,毛文轩,毛传澡

Identifying T-DNA insertion site(s) of transgenic plants by whole-genome resequencing

Xu Jiming,Hu Han,Mao Wenxuan,Mao Chuanzao

图2 3份转基因材料DNA和抗性基因PCR产物电泳检测结果 A:转基因株系PT8A-1、PT8A-2、PT8A-3基因组DNA电泳图。M:DL10000 marker。B:转基因株系PT8A-1、PT8A-2、PT8A-3 PCR产物电泳图。M:DL2000 marker。WT:野生型阴性对照;vector:35S-1300-G9A-PT8A阳性对照。

Fig. 2 Electrophoresis of the DNA extracted from three transgenic plants and PCR analysis of the resistant gene