RNA干扰猪NHEJ通路修复因子对HR效率的影响
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全绒,李国玲,莫健新,钟翠丽,李紫聪,顾婷,郑恩琴,刘德武,蔡更元,吴珍芳,张献伟
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Effects of RNA interference on the porcine NHEJ pathway repair factors on HR efficiency
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Quan Rong,Li Guoling,Mo Jianxin,Zhong Cuili,Li Zicong,Gu Ting,Zheng Enqin,Liu Dewu,Cai Gengyuan,Wu Zhenfang,Zhang Xianwei
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| 表1 合成siRNA序列信息 |
| Table 1 The sequence of siRNA |
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| 基因 | 编号 | 序列(5°→3°) | | 正向序列 | 反向序列 | | LIG4 | siRNA-1 | GCUAAGCUUUACAUCGAAUTT | AUUCGAUGUAAAGCUUAGCTT | | siRNA-2 | GGAAGCCCUUUCAUAGGAATT | UUCCUAUGAAAGGGCUUCCTT | | siRNA-3 | GCACUUUGCCCGGCAAUAUTT | AUAUUGCCGGGCAAAGUGCTT | | PNKP | siRNA-1 | CCUCGGGCGGGAUUUCCUUTT | AAGGAAAUCCCGCCCGAGGTT | | siRNA-2 | GGAAGCCGGUGAUCGGCAUTT | AUGCCGAUCACCGGCUUCCTT | | siRNA-3 | GCUCCCAAUGCCCAAUAAATT | UUUAUUGGGCAUUGGGAGCTT | | NHEJ1 | siRNA-1 | GGCUACAGCUUUCUGAGAATT | UUCUCAGAAAGCUGUAGCCTT | | siRNA-2 | GCCCGUUGUUGAAGGACAUTT | AUGUCCUUCAACAACGGGCTT | | siRNA-3 | GGUUGAAGACAGAGCCAUUTT | AAUGGCUCUGUCUUCAACCTT |
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