RNA干扰猪NHEJ通路修复因子对HR效率的影响

全绒,李国玲,莫健新,钟翠丽,李紫聪,顾婷,郑恩琴,刘德武,蔡更元,吴珍芳,张献伟

Effects of RNA interference on the porcine NHEJ pathway repair factors on HR efficiency

Quan Rong,Li Guoling,Mo Jianxin,Zhong Cuili,Li Zicong,Gu Ting,Zheng Enqin,Liu Dewu,Cai Gengyuan,Wu Zhenfang,Zhang Xianwei

表2 实时荧光定量PCR引物序列信息

Table 2 The primer sequences for qRT PCR

基因 序列(5°→3°) 产物片段 (bp) LIG4 F: GCCGCTATCGCAGACATTG 251 R: GCCATCATCTCACCATCAAGG PNKP F: GGACCGTGGCAGTGAAACAG 221 R: CTCTTCCTCCTCCTCGTGTGG NHEJ1 F: GCAGTGTTGGTGATGGAAGAC 176 R: TGGCTCCTCTGGCTGGTTC