Lnc-RAP3对小鼠3T3-L1前脂肪细胞分化的影响
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李欢,冯晋川,李贵林,王讯,李明洲,刘海峰
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The effect of lnc-RAP3 on 3T3-L1 preadipocyte differentiation in mouse
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Li Huan,Feng Jinchuan,Li Guilin,Wang Xun,Li Mingzhou,Liu Haifeng
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| 表1 本文所用的引物信息 |
| Table 1 Primers used in this study |
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| 基因 | GenBank登录号 | 引物序列(5°→3°) | 引物用途 | | lnc-RAP3 | NR_045146.1 | F: CCCAAGCTTTCCACCCTAGGAGGGTAAGG | 基因克隆 | | R: CGGGGTACCGGGGGAACCTTTATTTCAGGGA | | lnc-RAP3 | NR_045146.1 | F: ACACGAACACACGCATACAA | qRT-PCR | | R: TCATAACGACAGTGGTGCAG | | PPARγ | NM_011146.3 | F: AAGAGCTGACCCAATGGTTG | qRT-PCR | | R: ACCCTTGCATCCTTCACAAG | | C/EBPα | NM_001287523.1 | F: GGTTTTGCTCTGATTCTTGCC | qRT-PCR | | R: CGAAAAAACCCAAACATCCC | | Glut4 | AB008453.1 | F: CAGATCGGCTCTGACGATGG | qRT-PCR | | R: TCAATCACCTTCTGTGGGGC | | FAS | NM_007988.3 | F: GGAGGTGGTGATAGCCGGTAT | qRT-PCR | | R: TGGGTAATCCATAGAGCCCAG | | LPL | NM_008509.2 | F: AGGACCCCTGAAGACACAGCT | qRT-PCR | | R: TGTACAGGGCGGCCACAAGT | | ACTB | NM_007393.5 | F: CAGCCTTCCTTCTTGGGTAT | qRT-PCR | | R: TGGCATAGAGGTCTTTACGG |
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