利用CRISPR/Cas9敲除人源细胞系中LMNA基因的研究

刘恒,李东明,朱兰玉,赖乐锦,闫婉云,陆玉双,韦伊,黄月琪,方媚,苏元港,杨芳,舒伟

Research on the knockout of LMNA gene by CRISPR/Cas9 system in human cell lines

Liu Heng,Li Dongming,Zhu Lanyu,Lai Lejin,Yan Wanyun,Lu Yushuang,Wei Yi,Huang Yueqi,Fang Mei,Su Yuangang,Yang Fang,Shu Wei

表1 靶向人源LMNA基因敲除的gRNA序列

Table 1 Sequences of the targeting gRNA for the human LMNA knockout

名称 序列(5°→3°) FO1 TTCCGCCAGCAGCCGCCGGC RO1 GCCGGCGGCTGCTGGCGGAA FO2 AGCGGGAGATGGCCGAGATG RO2 CATCTCGGCCATCTCCCGCT FO3 CACGCAGCTCCTGGAAGGGT RO3 ACCCTTCCAGGAGCTGCGTG FO4 GCGCCGTCATGAGACCCGAC RO4 GTCGGGTCTCATGACGGCGC