利用CRISPR/Cas9敲除人源细胞系中LMNA基因的研究
Research on the knockout of LMNA gene by CRISPR/Cas9 system in human cell lines
利用CRISPR/Cas9敲除人源细胞系中LMNA基因的研究 |
| 刘恒,李东明,朱兰玉,赖乐锦,闫婉云,陆玉双,韦伊,黄月琪,方媚,苏元港,杨芳,舒伟 |
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Research on the knockout of LMNA gene by CRISPR/Cas9 system in human cell lines |
| Liu Heng,Li Dongming,Zhu Lanyu,Lai Lejin,Yan Wanyun,Lu Yushuang,Wei Yi,Huang Yueqi,Fang Mei,Su Yuangang,Yang Fang,Shu Wei |
| 图3 LMNA敲除细胞株和野生细胞株的细胞增殖能力和凋亡比较 A：细胞增殖曲线。LMNA敲除后细胞增殖能力都明显下降(n=3)。B：流式细胞仪检测细胞凋亡结果。LMNA敲除后细胞凋亡率都显著上升,FL1-H为绿光通道(Annexin V-FITC),FL3-H为红光通道(PI)。C：细胞凋亡的统计学分析(P<0.05, n=3)。293T WT为293T野生型细胞,293T KO为293T LMNA基因敲除细胞,HepG2 WT为HepG2野生型细胞,HepG2 KO为HepG2 LMNA基因敲除细胞。 |
| Fig. 3 Cell proliferation and apoptosis in LMNA knockout cell lines and its wild type |
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