利用CRISPR/Cas9敲除人源细胞系中LMNA基因的研究
Research on the knockout of LMNA gene by CRISPR/Cas9 system in human cell lines
利用CRISPR/Cas9敲除人源细胞系中LMNA基因的研究 |
| 刘恒,李东明,朱兰玉,赖乐锦,闫婉云,陆玉双,韦伊,黄月琪,方媚,苏元港,杨芳,舒伟 |
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Research on the knockout of LMNA gene by CRISPR/Cas9 system in human cell lines |
| Liu Heng,Li Dongming,Zhu Lanyu,Lai Lejin,Yan Wanyun,Lu Yushuang,Wei Yi,Huang Yueqi,Fang Mei,Su Yuangang,Yang Fang,Shu Wei |
| 图5 免疫荧光技术染色微丝观察LMNA敲除后细胞形态的改变 LMNA敲除后细胞骨架微丝的改变更为明显,蓝色为DAPI染色的细胞核,绿色为β-actin 293T WT为293T野生型细胞,293T KO为293T LMNA基因敲除细胞,HepG2 WT为HepG2野生型细胞,HepG2 KO为HepG2 LMNA基因敲除细胞。 |
| Fig. 5 Immunofluorescence staining microfilaments of LMNA knockout cell and its wild type |
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