利用CRISPR/Cas9系统构建人HPRT1基因定点突变细胞株

张楷,刘蔚,刘小凤,陈瑶生,刘小红,何祖勇

Generation of cell strains containing point mutations in HPRT1 by CRISPR/Cas9

Zhang Kai,Liu Wei,Liu Xiaofeng,Chen Yaosheng,Liu Xiaohong,He Zuyong

表1 CRISPR/Cas9基因编辑质粒Oligo序列

Table 1 The inserted sequence of CRISPR/Cas9 konckout plasmid

名称 gRNA序列(5°→3°) HPRT1 508-1 top oligo caccgAAAAGGACCCCACGAAGTGT HPRT1 508-1 bottom oligo aaacACACTTCGTGGGGTCCTTTTc HPRT1 508-2 top oligo caccGGCTTATATCCAACACTTCG HPRT1 508-2 bottom oligo aaacCGAAGTGTTGGATATAAGCC HPRT1 508-3 top oligo caccGCTTATATCCAACACTTCGT HPRT1 508-3 bottom oligo aaacACGAAGTGTTGGATATAAGC HPRT1 508-4 top oligo caccgCTTATATCCAACACTTCGTG HPRT1 508-4 bottom oligo aaacCACGAAGTGTTGGATATAAGc HPRT1 151-1 top oligo caccgTCTTGCTCGAGATGTGATGA HPRT1 151-1 bottom oligo aaacTCATCACATCTCGAGCAAGAc