利用CRISPR/Cas9系统构建人HPRT1基因定点突变细胞株
Generation of cell strains containing point mutations in HPRT1 by CRISPR/Cas9
利用CRISPR/Cas9系统构建人HPRT1基因定点突变细胞株 |
| 张楷,刘蔚,刘小凤,陈瑶生,刘小红,何祖勇 |
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Generation of cell strains containing point mutations in HPRT1 by CRISPR/Cas9 |
| Zhang Kai,Liu Wei,Liu Xiaofeng,Chen Yaosheng,Liu Xiaohong,He Zuyong |
| 图2 gRNA活性检测和切割效率验证 A：T7E1实验（红色箭头示T7E1酶切条带）;B：靶位点测序结果比对（红色字体示gRNA识别序列,黑色加粗字体示PAM位点）。 |
| Fig. 2 Detection of gRNA activity and knock out efficiency |
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