利用CRISPR/Cas9系统构建人HPRT1基因定点突变细胞株

张楷,刘蔚,刘小凤,陈瑶生,刘小红,何祖勇

Generation of cell strains containing point mutations in HPRT1 by CRISPR/Cas9

Zhang Kai,Liu Wei,Liu Xiaofeng,Chen Yaosheng,Liu Xiaohong,He Zuyong

图3 流式分选富集GFP阳性细胞提高突变频率和重组效率 A：转染24 h后荧光拍照;B：流式分选较强GFP阳性细胞;C：酶切实验(US: unsorted;S: sorted;T: T7E1 cleaved;N: NlaⅢ cleaved;B: BbsⅠ cleaved;M: 100 bp DNA marker);D：流式分选前后靶位点测序结果对比。

Fig. 3 Enhanced mutation rate and HDR efficiency with FACS