转录组测序揭示细胞周期通路参与鸡腹脂沉积
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陈家辉,任学义,李丽敏,卢诗意,程湉,谭量天,梁少东,何丹林,罗庆斌,聂庆华,张细权,罗文
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The cell cycle pathway regulates chicken abdominal fat deposition as revealed by transcriptome sequencing
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Chen Jiahui,Ren Xueyi,Li Limin,Lu Shiyi,Cheng Tian,Tan Liangtian,Liang Shaodong,He Danlin,Luo Qingbin,Nie Qinghua,Zhang Xiquan,Luo Wen
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| 表1 qRT-PCR扩增引物 |
| Table 1 The primer sequences of qRT-PCR used in this study |
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| 基因(NCBI登录号) | 引物序列(5?→3?) | | c-Myc (KU981087) | F:GCCAGCGAAGGAATGAGC | | R:CGTCCGATTGGATAGACAGAAC | | CDK1 (NM_205314.1) | F: AAGTGAGGAGGAAGGTG | | R: AATGGCAGAAGACGATA | | PLK1 (NM_001030639.1) | F: AACTGAACGGGCGAAAG | | R: GGGAATACAGGCTGGGTC | | CCNB3 (NM_205239.2) | F: TCCTCATCGCCTCCAAA | | R: CAGGGTCATCTCACAGACAAAA | | PTTG1 (XM_025154831.1) | F: GAAAGGCTCTTGGAAA | | R: TGAGGAGGTCTCGTTATT | | GADD45B (XM_015299957.2) | F: GCTTCGGTCCCTTTGGTGA | | R: CCGGCAGTTGTTGTGCAGTC | | CDC45 (XM_415070.6) | F: TGTCTCGCCATAACCA | | R: GCCGCTTCTGTCCTT | | CDKN2A (NM_204434.1) | F: TACGTTCTCCCTTCACCTCC | | R: AAACCGCTTCAACTGACTACAT | | CDKN2B (NM_204433.1) | F: AAACCCACCCGCTCTTCC | | R: CCGTCCCTTTCGGCTTCA | | β-actin (NM_205518.1) | F: TTGTTGACAATGGCTCCGGT | | R: AACCATCACACCCTGATGTCT | | GAPDH (NM_204305.1) | F: AGAACATCATCCCAGCGT | | R: AGCCTTCACTACCCTCTTG |
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