lncRNA TCONS_00815878对猪骨骼肌卫星细胞分化的影响

黄子莹, 李龙, 李倩倩, 刘向东, 李长春

The effect of lncRNA TCONS_00815878 on differentiation of porcine skeletal muscle satellite cells

Huang Ziying, Li Long, Li Qianqian, Liu Xiangdong, Li Changchun

表1 本文所用引物信息

Table 1 Sequences of primers used in this study

目的基因 引物序列( 5′→3′) 复性温度(℃) 产物大小(bp) TCONS_00815878 F: ACACCCTTTCCCAAAATCAA 56 93 R: GCAGACTGTCCAAATCTACCCT PFKM F: GAGAGCGTTTCCATGATGCTTC 60 221 R: AATCAAAGAGGGTGCCATCCAT ELOVL5 F: ATATGAAGATCATCCGCGTGCT 60 59 R: GTGATCTGGTGGTTGTTCTTGC MBTPS1 F: CCTCAACAGTGGTGGAATACGA 60 179 R: TTGGGATGATCTTCAAGCGTCA MyoD F: GGCTGCCCAAGGTGGAAATC 60 170 R: TGCGTCTGAGTCACCGCTGTAG MyoG F: ATGAGACATCCCCCTACTTCTACCA 60 160 R: GTCCCCAGCCCCTTATCTTCC Pax7 F: GGAGTACAAGAGGGAGAACCC 60 122 R: TTCTGAGCACGCGGCTAATC Myf5 F: GAATGCCATCCGCTACAT 60 125 R: AACTGCTGCTCTTTCTGG MyHC F: GTTCAGAGAAAGGCATCCCAAA 60 135 R: GAGAGTGACCGACACCACAAGTG FKBP10 F: ATGTGTCCTGGAGAGAGAAGGA 60 209 R: AATCAAAGAGGGTGCCATCCAT 18S rRNA F: TCCCGACGTGACTGCTC 60 133 R: GGTGACAGCGGGGTGG