鸡胰岛素降解酶基因环状转录本克隆及其表达规律
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冷奇颖, 郑嘉辉, 徐海冬, PatriciaAdu-Asiamah, 张颖, 杜炳旺, 张丽
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Cloning and expression analysis of chicken circular transcript of insulin degrading enzyme gene
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Leng Qiying, Zheng Jiahui, Xu Haidong, Adu-Asiamah Patricia, Zhang Ying, Du Bingwang, Zhang Li
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表2 引物序列信息
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Table 2 Sequences of the primers used in this study
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| 引物名称 | 引物序列(5°→3°) | 复性温度(℃) | 扩增产物长度(bp) | 位置 | 用途 | | circIDE-D | F: AGATAAAGAACGACCACG | 50 | 265 | Exon10 | 环状验证 | | R: CAATTCCAGTCCACGATA | Exon2 | | circIDE-full | F: TCTTCAGAAGGAGACAA | 46 | 1326 | Exon2 | 全长克隆 | | R: CGAATGTTTTCTGGTC | Exon11 | | circIDE-DL | F: GAAGAAGTATTGGCTGCTG | 54 | 198 | Exon11 | circIDE定量 | | R: TTCCAGTCCACGATATTCTC | Exon2 | | IDE-DL | F: TGGCGTATCTCAAGACCCTG | 57 | 175 | Exon22 | IDE mRNA定量 | | R: GCTGGTGCCAAGTTCACATC | Exon24 | | GAPDH | F: AGGACCAGGTTGTCTCCTGT | 57 | 153 | Exon7, 8 | 内参基因定量 | | R: CCATCAAGTCCACAACACGG | Exon9 |
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