鸡胰岛素降解酶基因环状转录本克隆及其表达规律

冷奇颖, 郑嘉辉, 徐海冬, PatriciaAdu-Asiamah, 张颖, 杜炳旺, 张丽

Cloning and expression analysis of chicken circular transcript of insulin degrading enzyme gene

Leng Qiying, Zheng Jiahui, Xu Haidong, Adu-Asiamah Patricia, Zhang Ying, Du Bingwang, Zhang Li

图2 鸡circIDE全长克隆及结构验证 A：circIDE接头位置扩增结果。M：DL2000 maker;泳道1~4：以1周龄正常体型芦花鸡肝脏组织cDNA为模板,circIDE-D引物扩增结果。B：circIDE-D引物扩增产物测序结果。C：circIDE全长扩增结果。M：DL2000 maker;泳道1：以1周龄正常体型芦花鸡肝脏组织cDNA为模板,circIDE-full引物扩增产物。D：鸡circIDE结构示意图。

Fig. 2 Cloning of the full length of chicken circIDE gene and validation of the circIDE structure