肺癌恶性胸腔积液中稀有肿瘤细胞的鉴定与单细胞测序分析

吴保军,王卓,董宇,邓宇亮,施奇惠

Identification and single-cell sequencing analysis of rare tumor cells in malignant pleural effusion of lung cancer patients

Wu Baojun,Wang Zhuo,Dong Yu,Deng Yuliang,Shi Qihui

表2 非小细胞肺癌EGFR基因检测扩增引物

Table 2 Primers of EGFR amplification for non-small cell lung cancer (NSCLC)

引物名称 序列（5'→3'） 片段大小(bp) EGFR18-F TGGAGAAGCTCCCAACCAA 231 EGFR18-R TTCCCAAACACTCAGTGAAACA EGFR19-F GTGGCACCATCTCACAATT 451 EGFR19-R ATGCTCCAGGCTCACCAAG EGFR20-F CTTTATCCAATGTGCTCCTC 486 EGFR20-R TCTCCCTTCCCTGATTACCT EGFR21-F TTCGCCAGCCATAAGTCCT 500 EGFR21-R TCATTCACTGTCCCAGCAAG