MEK抑制剂PD0325901显著提高猪胎儿成纤维细胞ssODN介导的HDR效率
|
|
欧浩,李国玲,王豪强,黄广燕,蔡更元,李紫聪,吴珍芳,张献伟
|
MEK inhibitor PD0325901 significantly boosts ssODN-mediated HDR efficiency in porcine fetal fibroblasts
|
|
Ou Hao,Li Guoling,Wang Haoqiang,Huang Guangyan,Cai Gengyuan,Li Zicong,Wu Zhenfang,Zhang Xianwei
|
|
| 表2 qRT-PCR引物序列信息 |
| Table 2 The sequences of quantitative real-time PCR primers used in this study |
|
| 基因 | 序列(5°→3°) | 产物大小
(bp) | | LIG4 | F：GCCGCTATCGCAGACATTG | 251 | | R：GCCATCATCTCACCATCAAGG | | PNKP | F：GGACCGTGGCAGTGAAACAG | 221 | | R：CTCTTCCTCCTCCTCGTGTGG | | XRCC5 | F：GAGGAAGGCACCGTTGAAG | 188 | | R：GAGAGAGGAATCTGACACTTAGC | | XRCC6 | F：GCGATGAAGAAGAAGAAGAGGAG | 225 | | R：CATAGAACACCACTGCCAAGAG | | BRCA1 | F：ACGCCACTCTCAACTTCTG | 195 | | R：CAAGCCTGATGCCACAATAG | | Rad52 | F：CTACTGGTGGCAACTCTGTATTATG | 163 | | R：ACCCTGTGACCCTCAATGTAAC | | Rad50 | F：GTGGTGATGCTAAAGGGAGAC | 232 | | R：GGAAGTTACGCTGCTGTGAG | | Rad51 | F：CGTTCAACACAGACCACCAG | 187 | | R：GCAAGTCGCAGAAGCATCC |
|
|
|