MEK抑制剂PD0325901显著提高猪胎儿成纤维细胞ssODN介导的HDR效率

欧浩,李国玲,王豪强,黄广燕,蔡更元,李紫聪,吴珍芳,张献伟

MEK inhibitor PD0325901 significantly boosts ssODN-mediated HDR efficiency in porcine fetal fibroblasts

Ou Hao,Li Guoling,Wang Haoqiang,Huang Guangyan,Cai Gengyuan,Li Zicong,Wu Zhenfang,Zhang Xianwei

表3 ssODN序列信息

Table 3 The sequences of ssODN used in this study

基因 序列(5°→3°) 长度(nt) EGFP CTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCG 140 DMD TTTATTGTTCAGCGTTTGGAATCTCCTGAAGACAAGTCATTTGGCAGTTCATTGTTGGAGACTGAAGTAAAAGCTTACCTGGACAGTTACCAAACAGCTTTAGAAGAAGTACTCTCATGGCTTCTTTCAGCTGAGGACACACTGCA 146 ROSA26 ATGTCTGGGACTGGATGAGCAAGTACAACAAACAAAATGGGCTTAAAGTATGAGTGAGAGTTATCTGACCAAGCTTGTAAGGATGCAAGTGAGGGGGCCTAAGGTTTGGAGATTAATATTTAATCTCAGATGCTATACTTTGGTGG 146