MEK抑制剂PD0325901显著提高猪胎儿成纤维细胞ssODN介导的HDR效率

欧浩,李国玲,王豪强,黄广燕,蔡更元,李紫聪,吴珍芳,张献伟

MEK inhibitor PD0325901 significantly boosts ssODN-mediated HDR efficiency in porcine fetal fibroblasts

Ou Hao,Li Guoling,Wang Haoqiang,Huang Guangyan,Cai Gengyuan,Li Zicong,Wu Zhenfang,Zhang Xianwei

表5 TA克隆数据统计

Table 5 The statistics of TA cloning

基因 浓度 KI效率(阳性/总数) DMD 对照组 18.75% (3/16) 250 nmol/L 27.78% (5/18) ROSA26 对照组 25.00% (4/16) 250 nmol/L 29.41% (5/17)