三代测序与靶向捕获技术联用进行高分辨HLA基因分型及MHC区域单倍体型精细鉴定

陈佳,舒明月,里进,付爱思,杨帆,王邹,李一荣,邓子新,刘天罡

The third-generation sequencing combined with targeted capture technology for high-resolution HLA typing and MHC region haplotype identification

Chen Jia,Shu Mingyue,Li Jin,Fu Aisi,Yang Fan,Wang Zou,Li Yirong,Deng Zixin,Liu Tiangang

表3 NGSengine和HLAminer的分型结果分辨率及与Illumina分型结果一致性统计

Table 3 Resolution of the typing results of NGSengine and HLAminer and their consistency with Illumina typing results

软件 分型分辨率6位 以上的基因个数 分型分辨率8位 以上的基因个数 与NGSengine-Illumina分型结果一致的基因个数 CCS0.80 CCS0.85 CCS0.90 CCS0.95 NGSengine 103/114 49/114 99/114 99/114 99/114 99/114 HLAminer 4/114 2/114 28/114 30/114 36/114 43/114