三代测序与靶向捕获技术联用进行高分辨HLA基因分型及MHC区域单倍体型精细鉴定

陈佳,舒明月,里进,付爱思,杨帆,王邹,李一荣,邓子新,刘天罡

The third-generation sequencing combined with targeted capture technology for high-resolution HLA typing and MHC region haplotype identification

Chen Jia,Shu Mingyue,Li Jin,Fu Aisi,Yang Fan,Wang Zou,Li Yirong,Deng Zixin,Liu Tiangang

表5 HCC27基于FALCON+FALCON-Unzip以及Targeted-phasing-consensus的单倍体分型结果

Table 5 The phasing results of HCC27 based on the FALCON+FALCON-Unzip and Targeted-phasing-consensus methods

FALCON+FALCON-Unzip Targeted-phasing-consensus contig_Tags 单倍体分型结果 consensus_Tags 单倍体分型结果 000009F|arrow A*02:01:01:01_gen consensus1 A*02:01:01:01_gen 000009F_001|arrow A*02:01:01:01_gen 000009F_001|arrow A*31:01:02:01_gen consensus0 A*31:01:02:01_gen 000018F|arrow B*46:01:01_gen consensus1 B*46:01:01_gen 000018F_002|arrow B*46:01:01_gen 000018F_002|arrow B*40:01:02:01_gen consensus0 B*40:01:02:01_gen 000018F|arrow C*01:02:01_gen consensus1 C*01:02:01_gen 000018F_002|arrow C*01:02:01_gen 000018F_002|arrow C*07:02:01:01_gen consensus0 C*07:02:01:01_gen 000027F_001|arrow DPA1*02:02:02_gen consensus0 DPA1*02:02:02_gen 000027F|arrow DPA1*04:01_nuc consensus1 DPA1*04:01_nuc 000027F_001|arrow DPB1*05:01:01_nuc consensus0 DPB1*05:01:01_nuc 000027F|arrow DPB1*13:01:01_nuc consensus1 DPB1*13:01:01_nuc 000017F|arrow DQA1*01:03:01:04_gen consensus0 DQA1*01:03:01:04_gen 000017F|arrow DQA1*03:02_gen consensus1 DQA1*03:02_gen 000017F|arrow DQB1*03:03:02:02_gen consensus1 DQB1*03:03:02:02_gen