染色质架构蛋白CTCF调控UGT1基因簇的表达

郑晓飞,黄海燕,吴强

Chromatin architectural protein CTCF regulates gene expression of the UGT1 cluster

Zheng Xiaofei,Huang Haiyan,Wu Qiang

表1 本研究使用的引物序列

Table 1 Primers sequences used in this study

类型 引物 序列(5′→3′) qPCR hCTCF F TCTGGACGACAATGAGGATGAG hCTCF R GCACCTGTATTCTGGTCTTCAAC 1A6F1 CAACTGTAAGAAGAGGAAAGAC 1A7F1 CCCCTATTTTTTCAAAAATGTCTT 1A9F1 GAACATTTATTATGCCACCG 1AR ATTGATCCCAAAGAGAAAACCAC hSMC3 F AATGATGTGATGAACCTCCTTG hSMC3 R TGAGAATCTGGTGCTGTTGC PCR C1F GGCTGCTCCTCCTATTTCCTG C1F2 GACCAGGACAAGGAGGCGT C1F5 TCTCCCCAGTGCTCCCATGT C1F8 AGGTTTGGGTGGAAAGAGGT C1R GCAAGTCTGCTTAGAGACTGAAGTG C1R2 ACCTGGGAGGGTGCTCTCA C1R6 CCTTAAAACTGTATGCATCCTAAGC C1R9 GTGAGGTCACCCCAGAAAAA sgRNA sgC1fF ACCGAATCATTCAGATCAGGCTAT sgC1fR AAACATAGCCTGATCTGAATGATT sgC1uF ACCGGGTCTAGGAGTCCTAGACGT sgC1uR AAACACGTCTAGGACTCCTAGACC sgC1dF ACCGTTAAACGTGTGCCTTTCTAA sgC1dR AAACTTAGAAAGGCACACGTTTAA shRNA pLKO.1-hCTCF-1F CCGGGAGGAGCCTGCCGTAGAAATTCTCGAGAATTTCTACGGCAGGCTCCTCTTTTTG pLKO.1-hCTCF-1R AATTCAAAAAGAGGAGCCTGCCGTAGAAATTCTCGAGAATTTCTACGGCAGGCTCCTC pLKO.1-hSMC3-1F CCGGGCAGTGCAACACAGAATTAAACTCGAGTTTAATTCTGTGTTGCACTGCTTTTTG pLKO.1-hSMC3-1R AATTCAAAAAGGCAGTGCAACACAGAATTAAACTCGAGTTTAATTCTGTGTTGCACTGC P7-index P7-index-wyh14 CAAGCAGAAGACGGCATACGAGATGCTCCGTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-wyh15 CAAGCAGAAGACGGCATACGAGATCATGAGAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-wyh18 CAAGCAGAAGACGGCATACGAGATCTGAATGCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-wyh20 CAAGCAGAAGACGGCATACGAGATTCGCATGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-wyh21 CAAGCAGAAGACGGCATACGAGATAATAGCAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-wyh22 CAAGCAGAAGACGGCATACGAGATGTCGCGTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT