Bmal1对小鼠胚胎期皮层神经元放射状迁移和轴突投射的影响
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李芳,黄青芸,刘斯佳,郭忠信,熊欣欣,桂林,束会娟,黄绍明,谭国鹤,刘媛媛
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The role of Bmal1 in neuronal radial migration and axonal projection of the embryonic mouse cerebral cortex
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Li Fang,Huang Qingyun,Liu Sijia,Guo Zhongxin,Xiong Xinxin,Gui Lin,Shu Huijuan,Huang Shaoming,Tan Guohe,Liu Yuanyuan
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| 表1 本研究所用引物信息 |
| Table 1 Primers used in this study |
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| 引物 | 序列(5°→3°) | 用途 | | Bmal1-shRNA-1# | F: GATCCCCGGAAGGATCAAGAATGCAATTCAAGAGATTGCATTCTTGATCCTTCCTTTTTA | 质粒构建 | | R: AGCTTAAAAAGGAAGGATCAAGAATGCAATCTCTTGAATTGCATTCTTGATCCTTCCGGG | | Bmal1-shRNA-2# | F: GATCCCCGCAACAGGCCTTCAGTAAATTCAAGAGATTTACTGAAGGCCTGTTGCTTTTTA | 质粒构建 | | R: AGCTTAAAAAGCAACAGGCCTTCAGTAAATCTCTTGAATTTACTGAAGGCCTGTTGCGGG | | Bmal1-shRNA-3# | F: GATCCCCGCGGAGGAAATCATGGAAATTCAAGAGATTTCCATGATTTCCTCCGCTTTTTA | 质粒构建 | | R: AGCTTAAAAAGCGGAGGAAATCATGGAAATCTCTTGAATTTCCATGATTTCCTCCGCGGG | | Bmal1引物 | F: GTGCCACCAACCCATACA | RT-PCR扩增 | | R: TCCCTCGGTCACATCCTAC | | Gapdh引物 | F: CCCCAATGTATCCGTTGTG | RT-PCR扩增 | | R: CTCAGTGT AGCCCAGGATGC |
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