拟南芥APX家族基因在植物生长发育与非生物逆境胁迫响应中的作用分析

李泽琴,李锦涛,邴杰,张根发

The role analysis of APX gene family in the growth and developmental processes and in response to abiotic stresses in Arabidopsis thaliana

Li Zeqin,Li Jintao,Bing Jie,Zhang Genfa

附表1 引物序列

Table 1 Supplemental Table 1 Primer sequences used in this study

引物名称 序列(5'→3') 用途 1F AGGAGGAAGCTCAGAGGTTTG apx1突变体鉴定 1R CAGCGTATTTCTCGACCAAAG 2F ATGAATCAAGCCCTTAAAGCC apx2突变体鉴定 2R TCAACCCTAACGTTTTTGTGC 3F CTTCATCCTCTTCCGGATCTC apx3突变体鉴定 3R TCCTGATGCCAAACAAGGTAC 4F GTAGGACAATCAGCGGTCAAG apx4突变体鉴定 4R TTGGAGGTGTTTCTCTGTTGG 5F CAGCTTCTCCTGATCCACTTG apx5突变体鉴定 5R TTTGAAGGTCCTTGGACACAG 6F TCAAATGGCAAGAACAGTGATC apx6突变体鉴定 6R CTTTTATCTGTTGGCGTGAGC sF TGATTGGTCAATTCAACCCTC sapx突变体鉴定 sR GCGTTAGCAGCATGTTTAAGC tF ACAAGATCAAACCCACGAATG tapx突变体鉴定 tR TACTTCACCAAGATGGGATGG LBb1.3 ATTTTGCCGATTTCGGAAC T-DNA插入位置检测 RT-1LP ACTACCCAACCGTGAGCGA 半定量RT-PCR检测APX1 RT-1RP ATCAGCGAAAAAGGCATCT RT-2LP GGAGTAAATGGTGAAGAAGAG 半定量RT-PCR检测APX2 RT-2RP TGAAAGTAAAAGAATGGTGTG RT-3LP TGGACCTAATGGCTCTATC 半定量RT-PCR检测APX3 RT-3RP TTGCTGCTTTTATTTCTTG RT-4LP CAATCATCAACATCTCACT 半定量RT-PCR检测APX4 RT-4RP AATCTCCTTCTTCACTTCC RT-5LP GCTAAACCGTCCACACAAC 半定量RT-PCR检测APX5 RT-5RP CCTCCACTGCAACAACTCC RT-6LP AAAGTGGTGACAAAGGGCA 半定量RT-PCR检测APX6 RT-6RP TCAGGGTTTCTGGAGGTAG RT-sLP GTCTCTCACACTCAACGGA 半定量RT-PCR检测sAPX RT-sRP ATGCCAACCTAATCTAACC RT-tLP TCTCACTTACTCTGTTCCT 半定量RT-PCR检测tAPX RT-tRP GCTTCATACTCTGCTCTTA ACT2A ACCTTGCTGGACGTGACCTTACTGAT 半定量RT-PCR检测ACTIN2 ACT2B GTTGTCTCGTGGATTCCAGCAGCTT 1A GTCCATTCGGAACAATGAGGTTTGAC qRT-PCR检测APX1 1B GTGGGCACCAGATAAAGCGACAAT 2A TGATGTGAAGACGAAGACAGGAGGAC qRT-PCR检测APX2 2B CCCATCCGACCAAACACATCTCTTA 引物名称 序列(5'→3') 用途 3A CCCAAAATCACATACGCAGACCTGTA qRT-PCR检测APX3 3B AGTTGTCAAACTTCAGCGGCTCTTG 4A CTACTAAATCCGGGGGAGCCAATG qRT-PCR检测APX4 4B CTCTGTTGCATCACTCCTTCCAAAAT 5A AGCTAAACCGTCCACACAACAAAGGT qRT-PCR检测APX5 5B GTCCCAAAGTGTGACCTCCAGAGAGA 6A TGCAAAACGAAATAAGGAAAGTGGTG qRT-PCR检测APX6 6B CACTCAGGGTTTCTGGAGGTAGCTTG sA TGCTAATGCTGGTCTTGTGAATGCTT qRT-PCR检测sAPX sB CCACTACGTTCTGGCCTAGATCTTCC tA CAGAATGGGACTTGATGACAAGGAAA qRT-PCR检测tAPX tB ATGCAGCCACATCTTCAGCATACTTC ACT2A ACCTTGCTGGACGTGACCTTACTGAT qRT-PCR检测ACTIN2 ACT2B GTTGTCTCGTGGATTCCAGCAGCTT ACT2C ATTCAGATGCCCAGAAGTCTTGTTCC qRT-PCR检测ACTIN2 ACT2D ACCACCGATCCAGACACTGTACTTCC