DNMT3a通过提升基因内部甲基化介导紫杉醇诱导的LINE-1异常表达

王昕源, 张雨, 杨楠, 程禾, 孙玉洁

DNMT3a mediates paclitaxel-induced abnormal expression of LINE-1 by increasing the intragenic methylation

Wang Xinyuan, Zhang Yu, Yang Nan, Cheng He, Sun Yujie

表2 甲基化特异性PCR所用引物

Table 2 The primers of methylation-specific PCR

引物名称 引物序列(5′→3′) LINE1- Promoter-MSP F: GTCGAATAGGAATAGTTTCGG R: ACTCCCTAACCCCTTACGCT LINE1-GB1- MSP F: GTTTAGATTTAGGAAATATAGAGAAC R: CTAACTTATAAAATTTCTACCGAA LINE1-GB2- MSP F: GTTGGATGGAGAATGATTTTGAC R: TTAATCACATCGACTCCTAAAAC Actin-MSP F: TGGTGATGGAGGAGGTTTAGTAAG R: AACCAATAAAACCTACTCCTCCCTTAA