DNMT3a通过提升基因内部甲基化介导紫杉醇诱导的LINE-1异常表达
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王昕源, 张雨, 杨楠, 程禾, 孙玉洁
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DNMT3a mediates paclitaxel-induced abnormal expression of LINE-1 by increasing the intragenic methylation
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Wang Xinyuan, Zhang Yu, Yang Nan, Cheng He, Sun Yujie
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表3 染色质免疫共沉淀所用引物
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Table 3 The primers of chromatin immunoprecipitation assays
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| 引物名称 | 引物序列(5′→3′) | | LINE-1-SP | F: TCACTAGGGAGTGCCAGACAG | | R: ATTTTCCAGGTGCGACCGTCA | | LINE-1-SGB1 | F: GTAGATAAAACCACAAAGATG | | R: TTGACGAGCTGAGAGAAGAAG | | LINE-1-SGB2 | F: GGAACGCAGTTCCTCACCAGC | | R: ATGTATAACTAGAATAACCAA | | LINE-1-SGB3 | F: GGCAAAGAAGTTGAAAACTTTG | | R: TCAGCTCCATCAGCTCCTTTA | | LINE-1-SGB4 | F: AAGGAGCTGATGGAGCTGAAA | | R: CTAAACTTCCCTTCTCGCTTCA | | LINE-1-SGB5 | F: CCGATGCGATCAACTGGAAGA | | R: TAAACTTCCCTTCTCGCTTCA | | LINE-1-SGB6 | F: CCAAGAAATATGGGACTATGT | | R: TAGATTGGGGAAGTTCTCCTG | | LINE-1-SGB7 | F: CAGGAGAACTTCCCCAATCTA | | R: CTGGCTGCCCTTAACATTT | | LINE-1-SGB8 | F: CAGATTCACCAAAGTTGAAATG | | R: CCACTCTCTTCTGGCTTGTAG |
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