内侧前额叶皮质DNA甲基化调控大鼠酒精相关行为
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崔亨贞, 孙蜜烛, 王润芝, 李辰雨, 黄予暄, 黄秋菊, 乔晓孟
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DNA methylation in the medial prefrontal cortex regulates alcohol-related behavior in rats
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Cui Hengzhen, Sun Mizhu, Wang Runzhi, Li Chenyu, Huang Yuxuan, Huang Qiuju, Qiao Xiaomeng
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表1 大鼠目的基因扩增引物序列信息
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Table 1 Primer sequences for target genes in rats
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| 基因名称 | 引物序列(5′→3′) | | Ntf3 | F: GGCAACAGAGACGCACAAT | | R: TCCTCCGTGGTGATGTTCTA | | Pdp1 | F: CTCAATTACCTGGTGCTTCG | | R: GCATTGTGGTCATTAGAGAGC | | Ppm1G | F: TCATTGCTTGTGACGGCATC | | R: ATGACAATAACCGAAGCTCCC | | FLT1 | F: TAACGCAATCTTCTTAACGTC | | R: TTAATCCTCACAAACGTCCC | | N1g2 | F: CAGAAGAGACAGAGCAGGCATC | | R: GCAGAGACCACCACATTCATC | | Hap1 | F: CAAGGATGAATGTGGTGGTCTC | | R: CTGCTGCCGCTTAGAATGG | | NFκB-p100 | F: TCGACCTCCACCGGATCTTT | | R: CTGTGTCTTTTGGCTTCGGC | | RTKN2 | F: CCATAGGATGCTAATCTCCGTTT | | R: AAGATTACTGCGGTCAACCTT | | DUSP1 | F: CATTCCCGATGACATACACGTT | | R: CATTCCCGATGACATACACGTT | | DUSP8 | F: TCACTGGTCTGGCTGGCATTTC | | R: AGTTGAAGTTGGGCGAGATGG | | DNMT1 | F: GTTCCTTGTAGGCGAGTGTG | | R: TTGCGTAGTCCTGGCTGTAC | | DNMT3A | F: GCA AAGTGAGGACCATTACC | | R: GCCAAACACCCTTTCCAT | | DNMT3B | F: GTGCGTCGTTCAGGCAGTAG | | R: GTTCTCGGCTCTCCTCATCT | | MeCP2 | F: GCCTTCAGCCCACCATTCTG | | R: CGGTCACGAATGATGGAACG | | GAPDH | F: ATGGGGAAGGTGAAGGTCG | | R: GGGGTCATTGATGGCAACAA |
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