早期停育胚胎的滋养层细胞相关基因与特征分析
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张悦, 冯颖, 马芳
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Related genes and characteristic analysis of trophoblast cells during early embryo developmental cessation
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Zhang Yue, Feng Ying, Ma Fang
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表2 实时荧光定量PCR引物序列表
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Table 2 Primer sequences used in quantitative real-time (qRT-PCR) validation
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| 序列 | 类型 | 基因名称 | 上游序列(5?→3?) | 下游序列(5?→3?) | | 1 | mRNA | C1QC | ATCCTGGGAAAAATGGCCCC | GAGGACCGCGTTGAATCTGA | | 2 | VSIG4 | TCCAGCAGGCAAAGTACCAG | GTTTCTGGACACGGAGCTCA | | 3 | SPP1 | GATGACCATGTGGACAGCCA | AACCACACTATCACCTCGGC | | 4 | CD36 | GACCGAGGAAGCCACTTTGA | TAAGCAGGTCTCCAACTGGC | | 5 | NCKAP1L | TGTCTTCCACTCCCGAATGC | TGCAGTGGACAAAGTGAGCA | | 6 | FGD2 | TGCTACGCATTCCTCACTGG | ATAGAGCACGAGGGGGTCAT | | 7 | LILRB5 | ACCCTGCTGTGTCAGTCATG | GTAGGACCTGATTGCGCTGT | | 8 | FOLR2 | GCACCACAAGACAAAGCCAG | CAGGTTGGGTGAGCACTCAT | | 9 | DPPA3 | CCAGGGTCTCCACAAATGCT | ATTTCCCTGAGGACTGCTGC | | 10 | MX1 | TCGGAGGCTACAGGAAGACT | TTTGCGATGTCCACTTCGGA | | 11 | lncRNA | CLRN1-AS1 | GAAAGTCTGAAGCCAGGCCT | CTTTGGGCTTGCACAGTCAC | | 12 | AC104809.4 | CGTGGGCTCGTCTAAGTGTT | GCACTGAGCTGTTTGCAGTC | | 13 | LINC01136 | ACCTCAGAGGCTACCCACAT | AGAAGAAATCCAGGGGCTGC | | 14 | USP27X-AS1 | TGCAACCAGAGGAACTGCAA | AGGTGGACCTATGGGCTTCT |
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