利用CRISPR/Cas9 AAV系统构建纹状体Slc20a2基因敲除小鼠模型
林珉婷, 赖璐璐, 赵淼, 林必玮, 姚香平

Construction of a striatum-specific Slc20a2 gene knockout mice model by CRISPR/Cas9 AAV system
Lin Minting, Lai Lulu, Zhao Miao, Lin Biwei, Yao Xiangping
Table 1 sgRNA sequence used in this study
引物名称 碱基序列(5?→3?)
sgRNA1 F:caccGCTCGTGGCGATTGGCCCGAA
R:aaacTTCGGGCCAATCGCCACGAGC
sgRNA2 F:caccGGCTTCTCACTCGTGGCGAT
R:aaacATCGCCACGAGTGAGAAGCC
sgRNA3 F:caccGTCGGGGACTCACTGCATCGT
R:aaacACGATGCAGTGAGTCCCCGAC