基于核质遗传原理建立多重PCR检测方法鉴定阿胶中马、驴源性成分及皮张种源

谌阳, 王文君, 付明, 徐国强, 周翔, 刘榜

A multiplex PCR method based on nuclear and cytoplasmic inheritance to identify the horse and donkey-derived components of Asini Colla Corii and the hide

Shen Yang, Wang Wenjun, Fu Ming, Xu Guoqiang, Zhou Xiang, Liu Bang

表1 扩增马、驴目的基因引物信息

Table 1 Primer sequences for target genes in horses and donkeys

目的基因 引物名称 序列(5?→3?) 目的片段大小(bp) OBSCN Horse-NF CAGGTGAGCCGAGCTGCGCT 166 Horse-NR TGGCGCACGCAGTTCGTCCC LOC106835644 Donkey-NF CTGTCCTGATGGAAGAGAACTTGG 144 Donkey-NR GGTGGTGCTGCAAATTCCACTTTA ND2 Horse-MF AAATCCCCTTATCTTCACAACT 179 Horse-MR GAAAATATTTGGTGGAGGCTTC ND2 Donkey-MF CATCCTACTAACTATAGCCGTG 130 Donkey-MR CACTAAGATGGCTGCTATTC