基于核质遗传原理建立多重PCR检测方法鉴定阿胶中马、驴源性成分及皮张种源
谌阳, 王文君, 付明, 徐国强, 周翔, 刘榜

A multiplex PCR method based on nuclear and cytoplasmic inheritance to identify the horse and donkey-derived components of Asini Colla Corii and the hide
Shen Yang, Wang Wenjun, Fu Ming, Xu Guoqiang, Zhou Xiang, Liu Bang
图4 马、驴二重PCR扩增体系特异性检测结果
A:马、驴核基因二重PCR特异性检测结果;B:马、驴线粒体基因二重PCR特异性检测结果。M:DNA Marker;1:空白对照;2:马;3:驴;4:马骡;5:驴骡;6~18:阴性对照(分别是普通牛、水牛、绵羊、山羊、猪、兔、狐、狗、貉、大鼠、小鼠、仓鼠和豚鼠)。
Fig. 4 Specific detection results of horse and donkey duplex PCR amplification