CRISPR-Cas基因编辑系统升级:聚焦Cas蛋白和PAM
唐连超, 谷峰

Next-generation CRISPR-Cas for genome editing: focusing on the Cas protein and PAM
Tang Lianchao, Gu Feng
图3 用于鉴定改变PAM特异性突变体的质粒干扰耗尽筛选
利用抗性的丢失来区分对PAM识别[33]
Fig. 3 Schematic of plasmid interference-based depletion screen to identify variants with altered PAM specificity