CRISPR-Cas基因编辑系统升级:聚焦Cas蛋白和PAM
唐连超, 谷峰

Next-generation CRISPR-Cas for genome editing: focusing on the Cas protein and PAM
Tang Lianchao, Gu Feng
图4 用细菌选择性定向进化进行正向选择
细菌中的可被识别PAM的毒性质粒被切割后,细菌得以存活[34]
Fig. 4 Schematic of the positive selection assay via bacterial selection-based directed evolution