乏情和发情初产母猪下丘脑-垂体-卵巢轴中lincRNAs表达谱比较分析

任巧玲, 张家庆, 陆东锋, 王璟, 陈俊峰, 马强, 白献晓, 郭红霞, 高彬文, 邢宝松

Comparison and analysis of lincRNAs expression profile in the hypothalamic-pituitary-ovarian axis of anestrous and estrous primiparous sows

Ren Qiaoling, Zhang Jiaqing, Lu Dongfeng, Wang Jing, Chen Junfeng, Ma Qiang, Bai Xianxiao, Guo Hongxiao, Gao Binwen, Xing Baosong

表2 引物序列信息

Table 2 The sequence information of primers

lincRNA 引物序列(5′→3′) 复性温度(℃) 扩增长度(bp) TCONS_00049640 F：TTCTCATAGTCCCCACACAGCC 60 177 R：CTTCTTCTTTCTCTTCCTTCCCC TCONS_00001098 F：TGAGGAAGAGCAGATAGCCAGTC 60 224 R：AGGTATTCAGGGAGGGCATCTA TCONS_ 00005732 F：TCCTTTCAGATTGGCTGGTGTT 60 230 R：CCACAGTCCAGGTCATCAAGC TCONS_00012288 F：GCCCAGTGGATTTCATTTGC 60 185 R：TGATACCATTCCTTCCTGTTCTCC GAPDH F：GTGAAGGTCGGAGTGAACGGA 60 252 R：CCATTTGATGTTGGCGGGAT