利用Trim-Away技术降低人胰岛淀粉样多肽在大鼠胰岛素瘤细胞中的毒性
|
|
龚葭薇, 孔德麟, 杨琳, 聂玉哲, 梁洋, 滕春波
|
Toxicity reduction of human islet amyloid polypeptide by Trim-Away technique in insulinoma cells
|
|
Gong Jiawei, Kong Delin, Yang Lin, Nie Yuzhe, Liang Yang, Teng Chun-Bo
|
|
表1 本研究使用的引物序列
|
Table 1 Primer sequences used in this study
|
|
| 目的 | 引物名称 | 序列(5'→3') | | PCR | lv3-hIAPP | F: acctccatagaagattctagaATGGGCATCCTGAAGCTGC | | R: ctctccatggtggcgtctagaTAAAGGGGCAAGTAATTCAGTGG | | pcDNA3.1-hIAPP | F: tagtccagtgtggtggaattcATGGGCATCCTGAAGCTGC | | R: tgctggatatctgcagaattcTAAAGGGGCAAGTAATTCAGTGG | | pcDNA3.1-humanTim21 | F: tagtccagtgtggtggaattcATGGCTTCAGCAGCACGC | | R: tgctggatatctgcagaattcATATAGTCAGTGGATCCTTGTGATCC | | pcDNA3.1-ratTrim21 | F: tagtccagtgtggtggaattcATGTCACACTCTACAACCTCAAAAATG | | R: tgctggatatctgcagaattcGGTATCTTTAGTGGACAGAGCTTTAGAG | | qPCR | Bax | F: TGTTACAGGGTTTCATCCAGG | | R: GTGTCCACGTCAGCAATCA | | Bcl2 | F: AAGCACATCCAATAAAAGCGC | | R: GTTATCATACCCTGTTCTCCCG | | Ins1 | F: CTAAGTGACCAGCTACAATCA | | R: CCACTTCACGACGGGACTTG | | Ins2 | F: TACAGTCGGAAACCATC | | R: GAAGGTCACCTGCCCCCG | | PDX1 | F: CCTACCCCGGGCTGCCGGCC | | R: CCGGGGGACTTCCCTGTTCC | | NeuroD1 | F: CTCTTTCAAACACGAACCATCC | | R: GAATGGTGAAACTGACGTGC | | Nkx6.1 | F: AGCACAAATCCAGCGGAG | | R: CTCCTTTCCTCGCCATCAC | | SLC2a2 | F: AGATTGCTCCAACCACACTC | | R: GCTTTTCTTTGCCCTGACTTC | | Actin | F: CAAGCAGGAGTACGATGAGTC | | R: AACGCAGCTCAGTAACAGTC |
|
|
|