CRISPR/Cas9系统在林木基因编辑中的应用

陈赢男, 陆静

Application of CRISPR/Cas9 mediated gene editing in trees

Chen Yingnan, Lu Jing

表1 CRISPR/Cas9系统在杨树中的应用

Table 1 Applications of CRISPR/Cas9 system in poplar

研究目的 杨树种名 基因 sgRNA 数目 Cas 9密码子 参考 文献 CRISPR 技术体系 的建立 与探索 毛白杨 (Populus tomentosa Carr. clone 741) PtoPDS 4 human codon-optimized Cas9 [6] 银灰杨 (P. tremula × alba clone 717-1B4) ; 欧美山杨 (P. tremula × tremuloides clone 353-38) LEAFY、AGAMOUS 1~2 human codon-optimized Cas9 [8] 银灰杨 (P. × canescens poplar clone INRA 717-1B4); 欧洲山杨 (P. tremula clone W52) SOC1、SOC1 Paralog 1、SOC1 Paralog 2、AGL8.1、AGL8.2、NFP-like1-4、TOZ19 1~2 Cas9 wild type gene (Streptococcus pyogenes) [9] 新疆杨 [P. alba var. pyramidalis (P. bolleana)] C2H2‐AZF 3 plant codon-optimized Cas9 [10] 木质素及 类黄酮 合成 银灰杨 (P. tremula × alba clone 717-1B4) 4CL1、4CL2 1 plant codon-optimized Cas9 [7] 毛白杨 (P. tomentosa Carr. clone 741) MYB115 3 plant codon-optimized Cas9 [14] 毛白杨 (P. tomentosa Carr. clone 741) PtoMYB156 3 plant codon-optimized Cas9 [15] 毛白杨 (P. tomentosa Carr. clone 741) PtoMYB170 3 plant codon-optimized Cas9 [16] 毛白杨 (P. tomentosa Carr. clone 741) PtrMYB57 3 plant codon-optimized Cas9 [17] 毛白杨 (P. tomentosa Carr. clone 741) JMJ25 1 plant codon-optimized Cas9 [18] 毛白杨 (P. tomentosa Carr. clone 741) MYB189 2 plant codon-optimized Cas9 [19] 木质部 发育 毛白杨 (P. tomentosa Carr. clone 741) PtoDWF4 3 plant codon-optimized Cas9 [20] 欧美山杨 (P. tremula L.× P. tremuloides clone T89) Pt×tvns09、vns10、 vns11、vns12 1 Arabidopsis codon-optimized Cas9 [12] 毛白杨 (P. tomentosa Carr. clone 741) PtoDET2 3 plant codon-optimized Cas9 [13] 生长与 生长 节律 银灰杨 (P. tremula × alba clone INRA 717-1B4) PcBRC1、PcBRC2 2 plant codon-optimized Cas9 [11] 银灰杨 (P. tremula × alba clone 717) GNC 1 Arabidopsis codon-optimized Cas9 [21] 银灰杨 (P. tremula × alba INRA clone 717-1B4) LHY2 1 human codon-optimized Cas9 [22] 欧美山杨 (P. tremula× tremuloides clone T89) BRC1 2 maize-codon optimized Cas9 [23] 抗旱性状 毛果杨 (P. trichocarpa) PtrADA2b-3 1 Arabidopsis codon-optimized Cas9 [24] 84K杨 (P. alba × P. glandulosa) PdNF-YB21 1 Arabidopsis codon-optimized Cas9 [25] 抗病性状 毛白杨 (P. tomentosa Carr. clone 741) PtrWRKY18、PtrWRKY35 3 plant codon-optimized Cas9 [26] 酚甙类次生 代谢产物 银灰杨 (P. tremula× alba INRA 717-1B4) UGT71L1 1 Arabidopsis codon-optimized Cas9 [27]