基于单细胞ATAC测序技术对18-三体综合征染色质开放性区域转录因子的分析

邱晓芬, 汤冬娥, 虞海燕, 廖秋燕, 胡芷洋, 周俊, 赵鑫, 何慧燕, 梁灼健, 许承明, 杨明, 戴勇

Analysis of transcription factors in accessible open chromatin in the 18-trisomy syndrome based on single cell ATAC sequencing technique

Qiu Xiaofen, Tang Dong’e, Yu Haiyan, Liao Qiuyan, Hu Zhiyang, Zhou Jun, Zhao Xin, He Huiyan, Liang Zhuojian, Xu Chengming, Yang Ming, Dai Yong

表2 qPCR的引物序列

Table 2 Primer sequences of qPCR

名称 序列(5'→ 3') 碱基数 H-TEAD1-F GCCACTGCCATTCATAACAAGC 22 H-TEAD1-R CCTGGCTGCCCTGTTTGAATC 21 H-TEAD2-F CCATTCTCACAGACACCGTTCAC 23 H-TEAD2-R TCCACGAAGGCTGAGAACTCTAC 23 H-TEAD4-F GACACGTACAACAAGCACCTG 21 H-TEAD4-R CCGTTCGAAGAGATCCTTGAGTC 23 H-Twist2-F CCCTCTGACAAGCTGAGCAAG 21 H-Twist2-R CATGCGCCACACGGAGAAG 19