不同基因型毛白杨同源重组变异研究

耿喜宁, 芦特, 杜康, 杨珺, 康向阳

Variation of homologous recombination in Populus tomentosa with different genotypes

Xining Geng, Te Lu, Kang Du, Jun Yang, Xiangyang Kang

附表1(续) 鉴定hDNA的110对SSR引物信息

Supplemental Table 1(Continued) The information of 110 pairs of SSR primers applied for detection of hDNA

标记名称 染色 体 位置 (Mb) 重复 单元 引物序列(5?→3?) Tm (℃) GC含 量(%) 3532中的 等位基因(bp) 3119中的 等位基因(bp) YX1中的 等位基因(bp) 标记名称 染色 体 位置 (Mb) 重复 单元 引物序列(5?→3?) Tm (℃) GC含 量(%) 3532中的 等位基因(bp) 3119中的 等位基因(bp) YX1中的 等位基因(bp) GCPM_1116-1 4 0.401 tca F: GGGGCTAATATCAGTTTCCT 53.35 45.00 195/201 195/201 195 R: TGAGAAAACCCTGGAAAATA 49.25 35.00 ORPM_394 4 0.667 tgc F: AAAAAGCCCCACAATTATTCA 49.76 33.33 222/225 222/225 222/231 R: GCAAGTTGCAATTGATGTCC 53.35 45.00 GCPM_3437 4 2.481 ag F: AAGATCTGGTGTTGTTTTGG 51.30 40.00 234/236 234/236 236/242 R: TTCTTAACAGAAGCCAGGAG 53.35 45.00 GCPM_3971-1 4 4.122 taaa F: CAGCCAGCCTCTTTAAGATA 53.35 45.00 193/211 193/211 202/220 R: ACAGCAATTCTGTACCTCGT 53.35 45.00 GCPM_1297-1 4 4.758 tct F: GGGATGAATCAGGAGATGTA 53.35 45.00 135/141 135/141 140 R: GAAGAAACCTGTGGGTGATA 53.35 45.00 ORPM_421 4 5.650 ta F: AAATGATGTTGCGATTTCCA 49.25 35.00 213/221 213/221 219 R: TCCCATCTCAACTACTCCAACA 55.81 45.45 PTSSR1738 4 6.603 ctcttc F: CTTCCTCGTTTGGCCCCTAA 57.45 55.00 277/295 277/295 289/301 R: ACCAGCTAGTTCGGGCTTTC 57.45 55.00 ORPM_201 4 7.736 tc F: GACTCCACCCAGTTCTGCTC 59.50 60.00 214/226 214/226 224/226 R: AACTTCCCATCGAATGATCG 53.35 45.00 PTSSR1873 4 8.381 GA F: GCGTGAGATGAGGGAGAGTG 59.50 60.00 184/198 184/198 186/202 R: CGTCAGGCGACAATTCCAAC 57.45 55.00 PTSSR1932 4 9.402 TAC F: CGGCAGGTCTGATGTCTTCA 57.45 55.00 120/123 120/123 117/123 R: CCATGGAGTCTAAAGGTGGCA 57.57 52.38 GCPM_3819-1 4 16.315 gaa F: ATCATCAAGTTCACGAAAGC 51.30 40.00 240/243 240/243 237 R: ACTGACCTACCACCATTTTG 53.35 45.00 GCPM_295-1 4 16.784 aag F: TAAGAGGATTACCAGGCAGA 53.35 45.00 136/145 136/145 142/148 R: CCCACTAACAATAAGCTTGG 53.35 45.00 PTSSR1623 4 20.046 tga F: CATCTCCCACCACCACAACA 57.45 55.00 238/248 238/248 238 R: CTCTGCGTAAACAACTGCGG 57.45 55.00 PTSSR2267 4 21.767 tca F: TGGAAGGCAAATCGTCGTCA 55.40 50.00 182/197 182/197 190 R: AAGTGGAGCAGACAGACACT 55.40 50.00 GCPM_3234-1 4 21.772 agc F: TCTCAATGTCGACACCATTA 51.30 40.00 195/198 195/198 195 R: TGCAAGAACTGCTATCACTG 53.35 45.00 GCPM_1255 5 1.974 ag F: GAACCTTAAAACCAGAACCC 53.35 45.00 191/193 191/193 189/193 R: GAGCCACAGAAATACTGCTC 55.40 50.00 GCPM_379-1 5 4.025 tat F: GGCCATTCATTAGATGTAGC 53.35 45.00 153/156 153/156 153 R: AAATCAAGATCCAAAGCAAA 47.20 30.00 GCPM_1063 5 5.996 ca F: AGTTAATTGCGCATGTTCTT 49.25 35.00 168/188 168/188 170/176 R: AAACAAACTCCAGCAAACAT 49.25 35.00 GCPM_3536-2 5 9.139 ttg F: AGATTCTTTTTCGGCTTCTT 49.25 35.00 137/146 137/146 141/144 R: AGAAGATGCTGGAGTTCAGA 53.35 45.00 GCPM_1838 5 9.156 ga F: GTTCAGCGAAAGCTAAAGAG 53.35 45.00 142/146 142/146 138/168 R: CACAGAATTACAGCTGATGC 53.35 45.00