不同基因型毛白杨同源重组变异研究

耿喜宁, 芦特, 杜康, 杨珺, 康向阳

Variation of homologous recombination in Populus tomentosa with different genotypes

Xining Geng, Te Lu, Kang Du, Jun Yang, Xiangyang Kang

附表1(续) 鉴定hDNA的110对SSR引物信息

Supplemental Table 1(Continued) The information of 110 pairs of SSR primers applied for detection of hDNA

标记名称 染色 体 位置 (Mb) 重复 单元 引物序列(5?→3?) Tm (℃) GC含 量(%) 3532中的 等位基因(bp) 3119中的 等位基因(bp) YX1中的 等位基因(bp) 标记名称 染色 体 位置 (Mb) 重复 单元 引物序列(5?→3?) Tm (℃) GC含 量(%) 3532中的 等位基因(bp) 3119中的 等位基因(bp) YX1中的 等位基因(bp) GCPM_2013-1 5 14.238 At F: TTTAGGGTCGTAGTGGAAAA 51.30 40.00 238/240 238/240 250/254 R: AAACCTGGAGGAGAATTAGAA 51.71 38.10 ORPM_136 5 16.608 ct F: TTTAAGCCTCCGAAAACCAA 51.30 40.00 228/240 228/240 232 R: CTGCAAGGCGAGGTTATTCT 55.40 50.00 ORPM_442 5 16.608 ct F: TGGTTTTCGGAGGCTTAAAA 51.30 40.00 244/254 244/254 236 R: CCTGCAGCGAGTATTAATTGG 55.61 47.62 GCPM_3151 5 22.434 ag F: ACCATCATTAACCCCACATA 51.30 40.00 144/156 144/156 135/155 R: AAAGAAACCAGACCACACAC 53.35 45.00 GCPM_2858-1 5 22.702 tg F: TGCAAGTCTTTTTAGGAACC 51.30 40.00 254/256 254/256 254/260 R: TTCAAAATGCATCAAAGTGT 47.20 30.00 GCPM_540-1 5 24.498 taa F: GATGGGGAGGTTATTTTCTT 51.30 40.00 154/160 154/160 154 R: CAATATTGAGGAAAATCAAAGG 50.22 31.82 GCPM_4008-1 5 24.561 acc F: AGAGAGAAGCTTGTGTCCAG 55.40 50.00 161/170 161/170 170 R: TGAGGAAGCAGAAGTAGAGC 55.40 50.00 ORPM_25 5 25.091 ta F: AAGAGTTGAAGGCTGGACGA 55.40 50.00 236/240 236/240 236 R: AGACATGCATGAAGCCATGA 53.35 45.00 GCPM_547-1 6 1.831 at F: CCTCTTGAAAAGAAGCAAAA 49.25 35.00 217/231 217/231 213 R: ATCAAAAATGCCGATTAAAA 45.15 25.00 GCPM_139 6 2.516 gt F: ATGACATGACATGATTGGAA 49.25 35.00 220/228 220/228 200/226 R: CTTCTGCTGGAAGAAGAAAA 51.30 40.00 GCPM_2126 6 3.888 ag F: CACGTAAACAGCTTCCAAGT 53.35 45.00 178/194 178/194 160/176 R: TAATGATTCCAGCTATGGGT 51.30 40.00 GCPM_1072-2 6 4.204 ttta F: AGGAAAACAAAGGAGAGGAG 53.35 45.00 150/154 150/154 142/154 R: ATGCTTAAAAGGGGATCTCT 51.30 40.00 GCPM_3539-2 6 5.331 aat F: TATTCGGTACAAGACTTGGG 53.35 45.00 215/218 215/218 212/215 R: TAATTGTAAAGCGGCTATCG 51.30 40.00 GCPM_2642-2 6 6.965 cca F: GCTTAGCTGGATGAGAAGAA 53.35 45.00 239/241 239/241 239 R: ATAGTTACAGGCCACCATTG 53.35 45.00 PTSSR580 6 13.716 atg F: AATGCATCCTCAGCTCCAGG 57.45 55.00 281/284 281/284 281 R: CGGCGGCTGCAAACATTAAT 55.40 50.00 GCPM_1017-1 6 22.438 ataa F: GTTTAATTCCCACGTCGTTA 51.30 40.00 171/175 171/175 175/177 R: CGAATGAAGAAAAACCATTC 49.25 35.00 GCPM_2705-1 6 22.633 ac F: TTTGCACAGGTAAGTTGATG 51.30 40.00 146/150 146/150 148/152 R: ATTGACCATAGCAGACAACC 53.35 45.00 GCPM_2034 6 23.087 ag F: ACAAACTGCTTTGTTTGGTT 49.25 35.00 176/190 176/190 186/196 R: CTCCATTCATAAAATCGAGC 51.30 40.00 GCPM_1632-1 6 23.663 ct F: TTTCTCTCTCTGAAACCCCT 53.35 45.00 211/225 211/225 203/221 R: AGCGACTCACTGAGCTTTAG 55.40 50.00 ORPM_365 6 23.788 tg F: GGGTTGGACCTGCTCAAATA 55.40 50.00 226/228 226/228 226/238 R: CAGCTGCTTTTTCATGGCTA 53.35 45.00