不同基因型毛白杨同源重组变异研究

耿喜宁, 芦特, 杜康, 杨珺, 康向阳

Variation of homologous recombination in Populus tomentosa with different genotypes

Xining Geng, Te Lu, Kang Du, Jun Yang, Xiangyang Kang

附表1(续) 鉴定hDNA的110对SSR引物信息

Supplemental Table 1(Continued) The information of 110 pairs of SSR primers applied for detection of hDNA

标记名称 染色 体 位置 (Mb) 重复 单元 引物序列(5?→3?) Tm (℃) GC含 量(%) 3532中的 等位基因(bp) 3119中的 等位基因(bp) YX1中的 等位基因(bp) GCPM_2627-1 6 24.930 cac F: TAAGTCCCACTACACCCAAC 55.40 50.00 234/254 234/254 240/255 R: GAGTTCGAGAGAGGGAATCT 55.40 50.00 GCPM_2615-1 6 27.782 cttt F: ATGTCAACGTCACTGACAAA 51.30 40.00 231/237 231/237 229/236 R: ATTAGGCAATGCAGAACACT 51.30 40.00 GCPM_1054-1 7 0.863 ct F: AGGTCTGTGCAAGGAATAAA 51.30 40.00 159/165 159/165 154/165 R: GTCTGTAATCAAGCCAAAGC 53.35 45.00 GCPM_741-1 7 1.416 at F: CCGTTTGATTAAAAAGATGC 49.25 35.00 212/214 212/214 214 R: TTATTGAGCTGATGATCCCT 51.30 40.00 WPMS_17 7 6.495 cac F: ACATCCGCCAATGCTTCGGTGTTT 59.57 50.00 137/146 137/146 137/140 R: GTGACGGTGGTGGCGGATTTTCTT 61.28 54.17 GCPM_3738-1 7 9.014 ac F: TGAACAAGACACCAAAATGA 49.25 35.00 133/139 133/139 133 R: TCTCGACTTTACCATCTCGT 53.35 45.00 GCPM_2741-1 7 12.076 at F: CAAGCAGTATCTTCCACTGA 53.35 45.00 142/146 142/148 134 R: TAGCCAACCACTCTAAAGGA 53.35 45.00 GCPM_3476-1 7 12.189 ggt F: GGGAATGTAAGGATGTGTTG 53.35 45.00 202/220 202/220 205/211 R: AACCAGAAAACGACAGTCAC 53.35 45.00 GCPM_3332-1 7 13.116 ag F: TCCACTGCCTATGAACTTTT 51.30 40.00 138/154 138/154 138 R: CACCCAATAGCTTCCATATT 51.30 40.00 GCPM_3727-1 7 13.848 ct F: TTGGGGTTAGTGACTAGTGG 55.40 50.00 152/158 152/158 152/154 R: CAAGCTGTGTAAAGACACCA 53.35 45.00 LG_Ⅷ-50 8 0.255 ga F: AACAACAGATCGCTGAAACT 51.30 40.00 265/281 265/281 265/271 R: CCATGTACTTAGGCATGGAT 53.35 45.00 GCPM_2992 8 1.833 TA F: GATCATAACCCAGGAAACAA 51.30 40.00 237/241 237/241 241 R: CGTGAATTTTTGGGATTTTA 47.20 30.00 GCPM_1344-2 8 3.669 tat F: GAGACCTGAGAAACAGAGGA 55.40 50.00 227/230 227/230 230 R: CAAATGCAACGCAATAATAA 47.20 30.00 GCPM_33-1 8 4.192 tta F: TTTGGCCTTAACTCTCCATA 51.30 40.00 171/174 171/174 167/179 R: AGTTGCTCTCAAAGAAGGTT 51.30 40.00 GCPM_13-1 8 7.309 taa F: ATTGTTCTTGTTGAAGGACG 51.30 40.00 207/213 207/213 204/210 R: AGAGCAAACAAATTGATGGT 49.25 35.00 ORPM_126 8 11.293 [ct]4cct F: GCCGAAGTTGACGATAGCTC 57.45 55.00 215/222 215/222 215 R: GAGTTAAACCCACCCTGCAA 55.40 50.00 ORPM_455 8 11.293 ga F: GAGTTAAACCCACCCTGCAA 55.40 50.00 215/225 215/225 215 R: GCCGAAGTTGACGATAGCTC 57.45 55.00 GCPM_2958-1 8 11.639 ct F: AAAGAGGATTACTTAGGCGG 53.35 45.00 201/215 201/215 217/219 R: ACGAGAACATTCATCAATCC 51.30 40.00 GCPM_3474-1 8 16.814 agc F: GATCCGAAAACAACAACAAT 49.25 35.00 123/135 123/135 123 R: ACCCCTTTCTCTTCTCAATC 53.35 45.00