不同基因型毛白杨同源重组变异研究

耿喜宁, 芦特, 杜康, 杨珺, 康向阳

Variation of homologous recombination in Populus tomentosa with different genotypes

Xining Geng, Te Lu, Kang Du, Jun Yang, Xiangyang Kang

附表1 鉴定hDNA的110对SSR引物信息

Supplemental Table 1 The information of 110 pairs of SSR primers applied for detection of hDNA

标记名称 染色 体 位置 (Mb) 重复 单元 引物序列(5?→3?) Tm (℃) GC含 量(%) 3532中的 等位基因(bp) 3119中的 等位基因(bp) YX1中的 等位基因(bp) LG_Ⅰ-18 1 0.047 atta F: CACGCTAAGATTGTCTCACA 53.35 45.00 320/332 320/332 320/328 R: TCACTGCATCAAGAAGATTG 51.30 40.00 LG_Ⅰ-22 1 0.059 agcag F: AGATAAGCATGGCTTGTTGT 51.30 40.00 309/314 309/314 309 R: CGTTACCATTCATTCCAAAT 49.25 35.00 GCPM_2935-2 1 0.275 ttc F: TCAGCTTCAGCTTCTTCTTC 53.35 45.00 192/204 192/204 198 R: AATTTGAAAAGGAAAAACCC 47.20 30.00 LG_Ⅰ-1223 1 1.241 gca F: ATATCACTCGCCAAGTCAAC 53.35 45.00 190/193 190/193 196/199 R: ATCACGCACAAGTTAGGTTT 51.30 40.00 LG_Ⅰ-1114 1 1.424 ta F: AAGTGGATCAGGTGATGAAG 53.35 45.00 156/166 156/166 165 R: CAATGTCTTTCGGTTCTTTC 51.30 40.00 LG_Ⅰ-1065 1 1.753 agc F: AATGGAGGTCCCTAGCTTAC 55.40 50.00 383/386 383/386 386 R: TGATATTGCTGGGCTATCTT 51.30 40.00 LG_Ⅰ-1027 1 1.834 ta F: TGAGCTTAGGAACGAAGAAG 53.35 45.00 188/198 188/198 184/186 R: CATCAATATGACTCCTCGGT 53.35 45.00 LG_Ⅰ-1125 1 1.835 tca F: GATGCTTACCCTTTGCTCTA 53.35 45.00 400/409 400/409 400 R: CATAATGGGCAAAGAATACC 51.30 40.00 GCPM_3979-1 1 4.749 ga F: TATCAATTTCCGTGGATTTC 49.25 35.00 192/196 192/196 193 R: GAAACTGAGAGGACTTGCTG 55.40 50.00 GCPM_124 1 6.328 cac F: TTTGAGCACTTCAACTACCA 51.30 40.00 207/210 207/210 201/210 R: TGTCTTCCCTTAGTCACCAC 55.40 50.00 GCPM_1960-1 1 11.783 gaaa F: TATCGAATCACAATGGCATA 49.25 35.00 220/224 220/224 220 R: TGCTCAGGTCATCTTTTCTT 51.30 40.00 GCPM_3408 1 20.668 gt F: ATGGAAGTTTGAGTCCACTG 53.35 45.00 202/212 202/212 200/202 R: CATGCATTACTTCAACCCTT 51.30 40.00 GCPM_2453-1 1 28.263 ggt F: ACACCAAGAGCTGTAGCATT 53.35 45.00 216/222 216/222 216/219 R: ACAACATGGCCTAACTCATC 53.35 45.00 GCPM_1719 1 36.636 aaag F: AAGTGCTCATAACATCACCC 53.35 45.00 216/228 216/228 227/234 R: CTTTCCTCATTCCTGTTCTG 53.35 45.00 GCPM_1263-1 1 41.934 ct F: TGCATTAAGACATCACTTGC 51.30 40.00 235/243 235/243 230/232 R: TTCGCTTCTGTATTTCCTGT 51.30 40.00 PTSSR1686 1 42.469 ttc F: TCAGGATCGGAGTCGGAGTT 57.45 55.00 165/168 165/168 165 R: TGAGCAGAAACGACACCACT 55.40 50.00 GCPM_734 2 1.595 cttt F: GGCAATTTAGGTACAACAGC 53.35 45.00 177/181 177/181 181 R: ACAAGCGAATGCTAATTGAT 49.25 35.00 GCPM_2085-1 2 1.957 ctt F: GCAGGAACTAACAAGGTGAG 55.40 50.00 154/157 154/157 154 R: TCGGTAGCAATTGTCTGAAT 51.30 40.00 GCPM_3588-1 2 2.443 aaca F: TCGACCAACTATGTTTGACA 51.30 40.00 184/184 184/184 191/195 R: AGTTGTAACTTTGGCCTGAA 51.30 40.00