鸡原始生殖细胞转染条件优化

邹娴, 何燕华, 何静怡, 王艳, 舒鼎铭, 罗成龙

Optimization of transfection conditions of chicken primordial germ cells

Xian Zou, Yanhua He, Jingyi He, Yan Wang, Dingming Shu, Chenglong Luo

表2 不同转染试剂和不同剂量GFP质粒转染PGCs后48 h的阳性细胞数(个/孔)

Table 2 Number of positive cells after 48 hours transfection of PGCs with different transfection reagents and different doses of GFP plasmid (cell/well)

Lipofectamine 2000	Lipofectamine 3000	Lipofectamine LTX & Plus Reagent
A1 (20.30±1.70)^b	B1 (50.40±7.10)^c	C1 (30.00±5.50)^c
A2 (40.50±3.50)^a	B2 (102.10±3.10)^b	C2 (55.90±4.40)^b
A3 (46.50±5.10)^a	B3 (278.50±10.90)^a	C3 (80.90±8.10)^a
A4 (40.70±10.80)^a	B4 (75.20±8.60)^b	C4 (63.30±9.20)^b