miR-191靶向BDNF基因通过激活PI3K/AKT信号通路促进猪未成熟支持细胞增殖
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唐湘薇, 楚丹, 颜赛娜, 尹艳飞, 卞桥, 翁波, 陈斌, 冉茂良
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MiR-191 promotes the porcine immature Sertoli cell proliferation by targeting the BDNF gene through activating the PI3K/AKT signaling pathway
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Tang Xiangwei, Chu Dan, Yan Saina, Yin Yanfei, Bian Qiao, Weng Bo, Chen Bin, Ran Maoliang
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表1 本研究所用的引物序列
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Table 1 Primer sequences used in this study
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| 基因 | 引物序列(5′→ 3′) | | CCND1 | F: TACACCGACAACTCCATCCG | | R: GCCGCCAGGTTCCACTT | | CCNE1 | F: CCTGCTGAAGATGCCCATAAC | | R: TGCTCTGCTTCTTACTGCTCG | | CDK-4 | F: GTGGCCCTCAAGAGCGTAAG | | R: CAGACATCCATCAGCCGGAC | | c-MYC | F: AACCCTTGGCTCTCCACGAG | | R: ATTCCGACCTTTTGGCAGGG | | FGF2 | F: ACCAGGTCACTGAGATCCATCCAC | | R: TTCGGCAACAGCACACCAATCC | | BMP4 | F: ACCAATCATGCCATCGTTCAGACC | | R: TGATTCAGCGGCAACCACATCC | | GDNF | F: GAGACCGCTGTGTATCGCATTCC | | R: GCCTTCTTCCTCTTCCTCCTCCTC | | PCNA | F: ATTTGGCCATGGGCGTGAAC | | R: CTAGTGCCAAGGTGTCTGCAT | | EGF | F: GCGAGCGATGTCAGCACAGAG | | R: AGGAGCAGCAGCAGGACCAG | | TBP | F: GCGATTTGCTGCTGTAATCA | | R: CCCCACCATGTTCTGAATCT |
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