miR-191靶向BDNF基因通过激活PI3K/AKT信号通路促进猪未成熟支持细胞增殖

唐湘薇, 楚丹, 颜赛娜, 尹艳飞, 卞桥, 翁波, 陈斌, 冉茂良

MiR-191 promotes the porcine immature Sertoli cell proliferation by targeting the BDNF gene through activating the PI3K/AKT signaling pathway

Tang Xiangwei, Chu Dan, Yan Saina, Yin Yanfei, Bian Qiao, Weng Bo, Chen Bin, Ran Maoliang

图2 miR-191抑制猪未成熟支持细胞凋亡 A：转染miR-191 mimic、mimic NC、miR-191inhibitor和inhibitor NC,流式细胞仪检测细胞凋亡情况;B：转染miR-191 mimic和mimic NC,细胞凋亡比例统计结果;C：转染miR-191 inhibitor和inhibitor NC,细胞凋亡比例统计结果;D：转染miR-191 mimic和mimic NC,细胞ATP水平;E：转染miR-191 inhibitor和inhibitor NC,细胞ATP水平;F：转染miR-191mimic和mimic NC,Western blotting 检测细胞凋亡标志基因Bcl2、BAX和Caspase-3蛋白表达水平;G：转染miR-191 inhibitor和inhibitor NC,Western blotting 检测细胞凋亡标志基因Bcl2、BAX和Caspase-3蛋白表达水平。*P<0.05表示差异显著,**P<0.01表示差异极显著。

Fig. 2 miR-191 impedes immature porcine Sertoli cell apoptosis