miR-191靶向BDNF基因通过激活PI3K/AKT信号通路促进猪未成熟支持细胞增殖

唐湘薇, 楚丹, 颜赛娜, 尹艳飞, 卞桥, 翁波, 陈斌, 冉茂良

MiR-191 promotes the porcine immature Sertoli cell proliferation by targeting the BDNF gene through activating the PI3K/AKT signaling pathway

Tang Xiangwei, Chu Dan, Yan Saina, Yin Yanfei, Bian Qiao, Weng Bo, Chen Bin, Ran Maoliang

图3 miR-191靶向BDNF基因3′-UTR A：miR-191与BDNF基因3′-UTR的潜在结合位点;B：共转染BDNF-WT + mimic NC、BDNF-WT + miR-191 mimic、BDNF-MUT + mimic NC和BDNF-MUT + miR-191 mimic后,检测双荧光素酶活性;C：转染mimic NC、miR-191 mimic、inhibitor NC和miR-191 inhibitor,qRT-PCR检测BDNF基因mRNA相对表达水平。*P<0.05表示差异显著,**P<0.01表示差异极显著。

Fig. 3 miR-191 targets the 3'-UTR region of theBDNF gene