miR-191靶向BDNF基因通过激活PI3K/AKT信号通路促进猪未成熟支持细胞增殖

唐湘薇, 楚丹, 颜赛娜, 尹艳飞, 卞桥, 翁波, 陈斌, 冉茂良

MiR-191 promotes the porcine immature Sertoli cell proliferation by targeting the BDNF gene through activating the PI3K/AKT signaling pathway

Tang Xiangwei, Chu Dan, Yan Saina, Yin Yanfei, Bian Qiao, Weng Bo, Chen Bin, Ran Maoliang

图7 miR-191靶向BDNF基因激活PI3K/AKT信号 A：转染miR-191 mimic和mimic NC,Western blotting检测p-PI3K和p-AKT蛋白相对表达水平及结果分析;B：转染miR-191 inhibitor和inhibitor NC,Western blotting 检测p-PI3K和p-AKT蛋白相对表达水平及结果分析;C：转染BDNF siRNA和siRNA NC,Western blotting检测p-PI3K和p-AKT蛋白相对表达水平及结果分析;D：共转染inhibitor NC + siRNA NC、miR-191 inhibitor + siRNA NC和miR-191 inhibitor + BDNF siRNA,Western blotting 检测p-PI3K和p-AKT蛋白相对表达水平及结果分析。*P<0.05表示差异显著,**P<0.01表示差异极显著。

Fig. 7 miR-191 activates PI3K/AKT signaling pathway by targeting the BDNF gene