串联反向CTCF位点的系列删除揭示增强子调控HOXD基因簇表达的平衡

王玲, 李金环, 黄海燕, 吴强

Serial deletions of tandem reverse CTCF sites reveal balanced HOXD regulatory landscape of enhancers

Wang Ling, Li Jinhuan, Huang Haiyan, Wu Qiang

表1 引物序列

Table 1 Primers used in this study

类型 引物名称 序列(5′→3′) PCR a1F TTCCAGCACCTCGGCTTTGTC a1R CCCACTTTCCACCTCTGTCCTG b1F GTCCGCCCGTGAGCTTCTGAA b1R1 CTCACAGCAGCCGAAACCG c1F TGATGCAGCCTCTGTGACCG c1R AGTTTTCCCGTGGCGTCTGA e1F TTCCCTGTCCCAGCTTGATTTC e1R TCAACAGTGAAGGGCGGTGC e2F CAAGCCACTCTCCCGCCACTA e2R TCGCTCTCGTCCTCTCTTGGG e2R1 GGCTCCTGCACTGAGACCACA sgRNA sgRNA a1F ACCGCGAAGAGTGCGGGAGAACGG sgRNA a1R AAACCCGTTCTCCCGCACTCTTCG sgRNA b1F ACCGGGCGCATCAGGAATGTAAG sgRNA b1R AAACCTTACATTCCTGATGCGCC sgRNA c1F ACCGCAGGCGAAGTGCGGTTTCCA sgRNA c1R AAACTGGAAACCGCACTTCGCCTG sgRNA e1F ACCGAACTGTGCTCAAACGCTCTC sgRNA e1R AAACGAGAGCGTTTGAGCACAGTT sgRNA e2F ACCGGAGGCGCAAACAGCTGTTGT sgRNA e2R AAACACAACAGCTGTTTGCGCCTC Index Island5-bioprimer 5′biotin-AAACACAAATGCATCAACCTG GT2-bioprimer 5′biotin-GAGCCAAACTGTACCCCTAGC HOXD9-bioprimer 5′biotin-ACCGACTAGTTCGCAGGCT Island5-P5 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGCATCTCATGAAGCTGGCATCT GT2-P5 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTAACTTTAGCTAAACCAAGGCCT HOXD9-P5 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGCAGCCTCCACCATTG P7-index-1 CAAGCAGAAGACGGCATACGAGATCGAGTAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-2 CAAGCAGAAGACGGCATACGAGATTCTCCGGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-3 CAAGCAGAAGACGGCATACGAGATAATGAGCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-4 CAAGCAGAAGACGGCATACGAGATGGAATCTCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-5 CAAGCAGAAGACGGCATACGAGATTTCTGAATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-6 CAAGCAGAAGACGGCATACGAGATACGAATTCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-7 CAAGCAGAAGACGGCATACGAGATAGCTTCAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-8 CAAGCAGAAGACGGCATACGAGATGCGCATTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-9 CAAGCAGAAGACGGCATACGAGATCATAGCCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-10 CAAGCAGAAGACGGCATACGAGATTTCGCGGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-11 CAAGCAGAAGACGGCATACGAGATGCGCGAGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-12 CAAGCAGAAGACGGCATACGAGATCTATCGCTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-13 CAAGCAGAAGACGGCATACGAGATAGAGTACTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-14 CAAGCAGAAGACGGCATACGAGATGCTCCGTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-15 CAAGCAGAAGACGGCATACGAGATCATGAGAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-16 CAAGCAGAAGACGGCATACGAGATTGAATCGCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-17 CAAGCAGAAGACGGCATACGAGATGTCTGACTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-18 CAAGCAGAAGACGGCATACGAGATCTGAATGCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-19 CAAGCAGAAGACGGCATACGAGATCGCTTCTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-20 CAAGCAGAAGACGGCATACGAGATTCGCATGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-21 CAAGCAGAAGACGGCATACGAGATAATAGCAGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-22 CAAGCAGAAGACGGCATACGAGATGTCGCGTAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-23 CAAGCAGAAGACGGCATACGAGATACGCGATAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-24 CAAGCAGAAGACGGCATACGAGATTGATCGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-25 CAAGCAGAAGACGGCATACGAGATCCGCATGAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-26 CAAGCAGAAGACGGCATACGAGATCCACAATCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT P7-index-27 CAAGCAGAAGACGGCATACGAGATGATGTTCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT