转基因玉米双抗12-5荧光RPA现场可视化检测方法的建立

陈欲, 陈笑芸, 彭城, 徐俊锋, 沈洁, 李玥莹, 汪小福

Establishment of a field visual detection method for genetically modified maize ‘Shuangkang’12-5 by fluorescence RPA

Chen Yu, Chen Xiaoyun, Peng Cheng, Xu Junfeng, Shen Jie, Li Yueying, Wang Xiaofu

表1 本文所用引物与探针序列

Table 1 Primer sequences and probe sequences used in this study

目的 名称 序列(5ʹ→3ʹ) 扩增产物长度(bp) 啊啊啊啊 荧光RPA 12-5-F1 TCCGACGCCTCGACGCTCGTGGTGACTCGC 本研究 12-5-F2 CGACCAAGTCATGAAGAACTCCCACTGCCG 12-5-F3 CGACCCGAAGATGGAGGCCTACTGCGATGA 12-5-F4 GACGTTCGGTGCCTGGAAGACAAGTTCTAC 12-5-F5 CGTCATCGACCAAGTCATGAAGAACTCCCA 12-5-F6 CAGCTCGTCATCGACCAAGTCATGA 12-5-F7 CCGACGCCTCGACGCTCGTGGTGACTCGCAGC 12-5-F8 CATCGACCAAGTCATGAAGAACTCCCACTGCCG 12-5-R1 ATGCTAGAGCAGCTTGAGCTTGGATCAGAT 12-5-R2 CCGATCGCCCTTCCCAACAGTTGCGCAGCC 12-5-R3 CCAGCTGGCGTAATAGCGAAGAGGCCCGCA 12-5-R4 TAATAGCGAAGAGGCCCGCACCGATCGCCC 12-5-R5 TGAATGGCGAATGCTAGAGCAGCTTGAGCT 12-5-R6 CTGAATGGCGAATGCTAGAGCAGCT 12-5-R7 CTTGAGCTTGGATCAGATTGTCGT TTCCCGCC 12-5-R8 CCCAACAGTTGCGCAGCCTGAATGGCGAATGC 12-5-P AACCACATCGCCCGACGCTACAACGAGAC[FAM-dT][THF]A [BHQ-dT]AGTTTAAACTGAA[3'-block] 普通PCR 12-5-PCR-F CAACGTCGTGACTGGGAAAA 256 [14] 12-5-PCR-R TGGAAGACAAGTTCTACGGGCT qPCR 12-5qPCR-F GTCGTTTCCCGCCTTCAGTT 94 12-5qPCR-R GGTGCCTGGAAGACAAGTTCTA 12-5qPCR-P [FAM]AGCTCAACCACATCGCCCGACGC[BHQ1]