转基因玉米双抗12-5荧光RPA现场可视化检测方法的建立
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陈欲, 陈笑芸, 彭城, 徐俊锋, 沈洁, 李玥莹, 汪小福
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Establishment of a field visual detection method for genetically modified maize ‘Shuangkang’12-5 by fluorescence RPA
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Chen Yu, Chen Xiaoyun, Peng Cheng, Xu Junfeng, Shen Jie, Li Yueying, Wang Xiaofu
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表2 荧光RPA和RT-PCR对实际样品的检测
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Table 2 The detection results of fluorescence RPA and real-time PCR
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| 样品 | 检测结果 | 检测时间(min) | | 荧光RPA | qPCR | 荧光RPA | qPCR | | 1 | + | + | 20 | 60.9 (Ct=27.95) | | 2 | + | + | 20 | 60.1 (Ct=27.55) | | 3 | + | + | 20 | 64.52 (Ct=29.76) | | 4 | + | + | 20 | 60.48 (Ct=27.74) | | 5 | + | + | 20 | 60.94 (Ct=27.97) | | 6 | + | + | 20 | 63.18 (Ct=29.09) | | 7 | + | + | 20 | 62.84 (Ct=28.92) | | 8 | + | + | 20 | 63.64 (Ct=29.32) | | 9 | + | + | 20 | 60.62 (Ct=27.81) | | 10 | + | + | 20 | 60.58 (Ct=27.79) | | 11 | + | + | 20 | 62.48 (Ct=28.74) | | 12 | + | + | 20 | 62.66 (Ct=28.83) | | 13 | + | + | 20 | 62.92 (Ct=28.96) | | 14 | + | + | 20 | 59.8 (Ct=27.40) | | 15 | + | + | 20 | 59.86 (Ct=27.43) | | 16 | + | + | 20 | 62.9 (Ct=28.95) | | 17 | + | + | 20 | 63.92 (Ct=29.46) | | 18 | + | + | 20 | 62.36 (Ct=28.68) | | 19 | + | + | 20 | 58.94 (Ct=26.97) | | 20 | + | + | 20 | 58.64 (Ct=26.82) | | 21 | + | + | 20 | 60.98 (Ct=27.99) | | 22 | + | + | 20 | 61.02 (Ct=28.01) | | 23 | + | + | 20 | 61.6 (Ct=28.30) | | 24 | + | + | 20 | 61.84 (Ct=28.42) | | 其他 | - | - | / | / |
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