小鼠生长激素受体基因环状转录本的克隆及其表达规律

张为露, 冷奇颖, 郑嘉辉, AliHassanNawaz, 焦振海, 王府建, 张丽

Cloning and expression of circular transcript of mouse growth hormone receptor gene

Zhang Weilu, Leng Qiying, Zheng Jiahui, Hassan Nawaz Ali, Jiao Zhenhai, Wang Fujian, Zhang Li

表1 小鼠8个circGHR的特异性验证引物

Table 1 Eight specific verification primers for mouse circGHR

引物名称 引物序列(5ʹ→3ʹ) 复性温度(℃) 扩增产物长度(bp) 位置 用途 circGHR1 F：TAGTTTGACCGGGATTCGTGG 59 171 Exon7 circGHR1 环状验证 R: GGAACGACACTTGGTGAATCG Exon4 circGHR2 F：GGTGAGATCCAGACAACGGA 59 134 Exon8 circGHR2 环状验证 R: GACACTTGGTGAATCGAGGC Exon4 circGHR3 F：GGGATTCGTGGAGACATCCAA 59 343 Exon7 circGHR3 环状验证 R: GACTGCCAGTGCCAAGGTTA Exon2 circGHR4 F：GACCGGGATTCGTGGAGACATC 61 422 Exon7 circGHR4 环状验证 R: ACGACACTTGGTGAATCGAGG Exon4 circGHR5 F：CCATCCCATATGGTGGATCTGT 59 142 Intron1 circGHR5 环状验证 R: ATGGGAAAGGAGGTGATGGC circGHR6 F：GCTGGACCAAAAATGTTTCACTGTT 60 176 Exon6 circGHR6 环状验证 R: CGTTGGCTTTCCCTTTTAGCA Exon4,5 circGHR7 F：CAGCGAAGTCCTCCGTGTAATA 59 100 Exon8 circGHR7 环状验证 R: CAGGGCATTCTTTCCATTCCTG Exon6 circGHR8 F: CAGTCACCAGCAGCACATTTT 58 70 Exon2 circGHR8 环状验证 R: AGGTTAAGAAGACCTGACAAAGAT