拟南芥ABI5基因对BR胁迫响应及其对下胚轴生长的调节作用
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林建辉, 刘自广, 张楠, 严容, 张楠, 何鑫淼, 王文涛, 刘娣, 吴娟
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Stress response of ABI5 to BR stress and its regulation on hypocotyls growth in Arabidopsis thaliana
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Lin Jianhui, Liu Ziguang, Zhang Nan, Yan Rong, Zhang Nan, He Xinmiao, Wang Wentao, Liu Di, Wu Juan
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表1 本研究所用引物信息
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Table 1 Information on primers used in this study
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| 引物名称 | 引物序列(5ʹ→3ʹ) | 复性温度(℃) | 用途 | | Actin2-F | GGTAACATTGTGCTCAGTGGTGG | 58 | qRT-PCR | | Actin2-R | AACGACCTTAATCTTCATGCTGC | | abi5-1-F | CGAGGGTGGTGTTGGTGTCTTTA | 60 | 突变体鉴定 | | abi5-1-R | CTACTCCATACTGACCTCCTA | | ABI5-F(q) | AACCTAATCCAACCCGAACC | 60 | qRT-PCR | | ABI5-R(q) | ACCCTCCTCCTCCTGTCC | | BIN2-F(q) | ACAAAAGGATGCCCCCAGAA | 59 | qRT-PCR | | BIN2-R(q) | TGAAGTTGAAGAGAGGCGGG | | BES1-F(q) | GCAATTGTCTCCAAACACAGCAG | 61 | qRT-PCR | | BES1-R(q) | CTCCAATCCTTCCTTCCGACATG | | BZR1-F(q) | GCAGATGTCTCCAAATACTGCTG | 61 | qRT-PCR | | BZR1-R(q) | GACATGCCATTTGGGTTTGCCTAG | | PIN7-F(q) | GTGGGATGTGGCAATGCCTAA | 60 | qRT-PCR | | PIN7-R(q) | TCCAATAGCCATTGCTGCCAC | | EXP3-F(q) | GGAACTTGTACAGCCAAGGATA | 57 | qRT-PCR | | EXP3-R(q) | AATAGATGGATTTCCCGGAACA |
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